Open Access
Copyright: The authors. This article is an open access
article licensed under the terms of the Creative Commons Attribution License
(http://creativecommons.org/licenses/by/2.0) which permits unrestricted use,
distribution and reproduction in any medium, provided the work is properly
cited.
Original Research
4.
Computer assisted semen analysis for quantification
of motion characteristics of bull sperm during
cryopreservation cycle -
M. N. Sundararaman, J Kalatharan, K Thilak Pon
Jawahar
Vet World. 2012; 5(12): 723-726
doi:
10.5455/vetworld.2012.723-726
Abstract
Aim:
The study was undertaken to quantify and to analyze
the changes in the motion characteristics of bull
spermatozoa during various stages of
cryopreservation cycle.
Materials and Methods:
Using computer assisted semen analysis (CASA)
technique, 26 ejaculates, collected from two Jersey
bulls were analyzed for motility, head behaviour and
swimming pattern of spermatozoa on dilution,
pre-freeze and post-thaw stages of cryopreservation.
French straw technique was employed for
deep-freezing of semen using liquid nitrogen.
Results: Equilibration of diluted semen at 5OC
has significantly (P< 0.01) reduced sperm motility,
progressive motility, path velocity, and progressive
velocity. Beat cross frequency was also affected
significantly (P<0.05) by equilibration. Freezing
and thawing processes drastically affected all the
motility, velocity and head behaviour
characteristics (P< 0.01).
Conclusion: CASA
facilitate objective evaluation sperm motion
characteristics. Adoption of CASA technique has the
potential for improvements in evaluation of semen
thereby the quality of frozen semen for fertility
can be enhanced.
Keywords: bull,
cryopreservation, CASA, spermatozoa
